Western blot analysis of rabbit anti-GAPDH antibody (ET1601-4,1/2000) and rabbit anti-GST3 antibody (ER1802-63, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10 µg per lane). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (HA1031) was used for 1 hour at room temperature at 1:40,000 (Lane A, E), 1:80,000 (Lane B, F), 1:160,000 (Lane C, G) and 1:320,000 (Lane D, H).

Exposure time: 30 seconds

The Cross-reactions of the secondary antibody (HA1031) with Rabbit IgG, Mouse IgG and Human IgG.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Alpaca anti-Rabbit IgG FC, Recombinant VHH (HRP) (HA1031) was used for 1 hour at room temperature at 1/50,000.

Lane 1: Rabbit IgG (50 ng/Lane)
Lane 2: Mouse IgG (50 ng/Lane)
Lane 3: Human IgG (50 ng/Lane)

Exposure time: 10 seconds.

Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using rabbit anti-Cytokeratin 18 antibody.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-8, 1/200 dilution) for 30 minutes at room temperature. Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (HA1031, 1/200 dilution) was used for 30 min at room temperature. Tissues were counterstained with hematoxylin and mounted with DPX.

The inset negative control image is secondary-only at 1/200 dilution.

Immunohistochemical analysis of paraffin-embedded human Kidney tissue using rabbit anti-CD13 antibody.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-45, 1/200) for 30 minutes at room temperature. Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (HA1031, 1/200 dilution) was used for 30 min at room temperature. Tissues were counterstained with hematoxylin and mounted with DPX.